Chukwunonso Chuka Nnabude

Preparation of DNA libraries for NEXT GENERATION SEQUENCING with manual and automated methods

DNA purification, quantification and quality control using various methods e,g electrophoresis, fluorometry.

Planning and Documentation of work process in LIMS

Laboratory organization and planning according according to ISO15189

Supporting in Equipment validation and process optimization

• Extraction and analysis of lipids by thin layer chromatography
• Expression of the desired protein in Dictyostelium cells
• Verification of the expressed protein by SDS and Western blot
• Fluorescence microscope on fixed Dictyostelium cells
• Scientific writing and report presentation

• Monitoring and evaluation of health projects
• Analysis and Evaluation of Data in tables and diagrams
• Writing scientific reports Results and generating grant proposals
• Presentation in team meeting

• Investigation of the MAST 1 gene in D
• Melanogaster
• Ligation of the MAST 1 gene with plasmid DNA vector
• Isolation and purification of plasmid DNA
• Point mutation of the MAST 1 gene by PCR
• Cloning of the mutated MAST 1 gene in E
• Coli
• Plasmid DNA digestion using various restriction enzymes
• Plasmid DNA size determination by agarose gel Electrophoresis

• Synthesis of phosphate polymers (silacidine analogue)
• Phosphate determination by UV spectroscopy
• Determination of the functional group by IR spectroscopy
• Determination of the functional group by 1H-NMR/ 31P- NMR
• Molecular weight determination using GPC

• Investigation of proteins involved in lipid metabolism in amoebae
• Isolation and knockout of the genes encoding these proteins
• Ligation of the mutated DNA with plasmid DNA vectors
• Amplification of mutated DNA with PCR
• Cloning of the mutated DNA
• Maintenance of D
• Discoideum cell cultures

• Counseling patients and maintenance of patients Data
• Therapeutic drug monitoring and evaluation
• Monitoring, control and sales of pharmaceutical and vaccine with 60 percent increased turn over
• Costs and dispensing of prescriptions

• Compounding and dispensing of pediatric prescriptions and dosages
• Therapeutic drug monitoring of inpatients
• Seminars and lectures on the pathophysiological aspects of chronic diseases
• Costs and dispensing of prescriptions

• Isolation and purification of endophytic fungi
• Characterization of chemical compounds